Reporter
mRFP sel.

Part:BBa_K1362422:Design

Designed by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha   Group: iGEM14_Heidelberg   (2014-10-07)


mRFP selection marker with outward BsaI restriction sites


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 788
    Illegal AgeI site found at 900
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1077
    Illegal BsaI.rc site found at 2


Design Notes

This part will not be sent as physical DNA. It is however a useful part for golden gate cloning.


Source

This selection marker part comes from the iGEM team Heidelberg 2014's Intein Toolbox [1]. The associated cloning strateges can be reviewed in RFC[???]. The mRFP generator sequence was cloned from BBa_J04450.

References